Skhep1 cells in culture have been shown to produce fibronectin and functionally active alpha1 protease inhibitor. Ac3, a novel marker for human hematopoietic stem and. Skhep1 cell line was reported to be endothelial origin. To verify this hypothesis, we explored the role of dab2 in vegf receptor endocytosis and lsec dedifferentiation in skhep1 human liver sinusoidal endothelial cell. This cell line is capable of forming tumors in immunocompromised mice. Lycopene inhibits reactive oxygen species production in sk.
Genomic alterations across six hepatocellular carcinoma cell lines. Immortalization of human fetal sinusoidal liver cells by polyoma virus large t antigen. The y chromosome could not be detected in this cell line by short tandem repeat strpcr analysis when tested at ecacc. Therefore, an alternative mechanism may be associated with the expression of. A novel monoclonal antibody to fibroblast growth factor 2. Sk cell is a human cell line derived from a patient with liver adenocarcinoma in 1971 11, and it has been reported to be of endothelia origin with. Sk hep1 is an immortal, human hepatic adenocarcinoma cell line that grows in adherent culture. Human liver adenocarcinoma cell line description derived from an ascites sample from a 52 year old male suffering from adenocarcinoma of the liver. Hepg2 is often used as a tool for screening the cytotoxicity of novel compounds because it is well differentiated and shares many of the genotypic features of normal hepatocytes 17,18. Further, the ability of the skhep1 cells to adopt the shape of the substrate pattern indicates they are more structurally labile, which may contribute to their higher mobility.
The cells have now been shown to be endothelial in origin in vitro 1992. In human endothelial cells, other growth factors, such as basic fibroblast growth factor, vegf, and hepatocyte growth factorscatter factor hgfsf, can induce the expression of cd44. Skhep1 atcc htb52 homo sapiens liver ascites adenoc. Sk hep1 cells sk cells derived from a patient with liver adenocarcinoma have been considered a human hepatoma cell line with mesenchymal origin characteristics, however, sk cells do not express liver genes and exhibit liver function, thus, we hypothesized whether mesenchymal cells might contribute to human liver primary cancers. Us8969041b2 large scale and stable production of human. Read lycopene inhibits reactive oxygen species production in skhep1 cells and attenuates acetaminopheninduced liver injury in c57bl6 mice, chemicobiological interactions on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. This leads to deregulation of host cell cycling, which in turn creates an environment favorable for viral replication. Cells were treated with or without dimethyl sulfoxide dmso vehicle control or compound 1 figure 1 or 2 figure 2 at concentrations of 1, 2, 5, or 10. Derived from an ascites sample from a 52 year old male suffering from adenocarcinoma of the liver. Description skhep1 is an immortal, human hepatic adenocarcinoma cell line that grows in adherent culture. Cobaltii diphenylazodioxide complexes induce apoptosis.
We investigated the concentrationdependent changes in the skhep1 cell morphology induced by cobaltii azodioxide complexes. Primary ec have a limited lifespan and display characteristics that differ from batch to batch due to their multidonor origin. The response of fenestrations, actin, and caveolin1 to. Galf2 had a substantial inhibitory effect on skhep1 xenografts fig. The y chromosome could not be detected in this cell line by short tandem repeat strpcr analysis when tested at. The results revealed that skhep1 cells expressed endothelialspecific makers. Serum autoantibodies to cell surface determinants in. Preclinical efficacy testing for stomach and liver cancers. In contrast to hepg2 cells, the sk hep1 cell line does not exhibit hepatocyte cell properties 31 despite the location where the tumor was derived. Sk hep1 cells are originally derived from endothelial cells, which express cd44. Hla a2, a9, b, b17 htb52 skhep1 liverascites endothelial adenocarcinoma homo sapiens 52 years caucasian.
Despite the location of the tumor from which sk hep1 was derived, the. The skhep1 cell line was proved by heffelfinger et al. Proteomic analysis reveals dab2 mediated receptor endocytosis. Miriam martins chaves from the federal university of minas gerais, brazil. Background sk hep1 cells sk cells derived from a patient with liver adenocarcinoma have been considered a human hepatoma cell line with mesenchymal origin characteristics, however, sk cells do not express liver genes and exhibit liver function, thus, we hypothesized whether mesenchymal cells might contribute to human liver primary cancers. Skhep1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. Skhep1 has an endothelial origin and expresses both vascular endothelial growth factor receptor2 and fibroblast growth factor receptor 1. Finally, we also tested galf2 against the skhep1 cell line, which is widely considered to be an hcc line, although it seems to be from a liver adenocarcinoma of endothelial origin. The cancer cell line skhep1, but not normal human umbilical vein endothelial cells, responded to highmacromolecularcontent solutions by elongating and. The disease relevance of human hepatocellular xenograft. The technical object of the present patent application is intended for the cultivation of human cells in suspension and in adhesion and.
Based on this, we hypothesized that regulatory proteins from human papillomaviruses hpvs can. Purchase skhep1 cell line for research and science from. Endothelial cells ec are currently used as in vitro model systems for various physiological and pathological processes, especially in angiogenesis research. A total of six human hepatoma cell lines were used. Background sk hep1 cells sk cells derived from a patient with liver adenocarcinoma have been considered a human hepatoma cell line with mesenchymal origin characteristics, however, sk cells do. Indeed, skhep1 transfectants expressing similar levels of cx37319p and cx37319s displayed a clear difference in proliferation after 48 h of culturing t48, whereas the number of cells initially plated t0 were equal figure 1c. Read expression of mutant amyloid precursor proteins decreases adhesion and delays differentiation of hep1 cells, brain research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Human papilloma virus e6 and e7 proteins support dna. Finally, the growthreducing effect of cx37319p was confirmed in skhep1 cells, a human endothelial cell line. In the present study, we examined the ultrastructural changes associated with denv2 replication in three susceptible host cells, c636, vero and sk hep1, a cell line of human endothelial origin. Addition of basement membrane material matrigel or type i collagen to the cultures induces these cells to organize into a tubular network. Despite the location of the tumor from which sk hep1 was. This is a list of cell lines that have been crosscontaminated and overgrown by other cells. Pdf hepatoma sk hep1 cells exhibit characteristics of.
Induction of apoptosis and growth suppression by homeobox gene tgiflx in prostate cancer cell line lncap. Evidence for insulinlike growth factor igfindependent transcriptional regulation of igf binding protein3 by growth hormone in skhep1 human hepatocarcinoma cells. Sh was also significantly higher in multiple sclerosis than non. Retinoblastoma cell line expression led us to examine ac3 expression on other cell lines of neural origin. The skhep1 cell line derived xenograft cdx, with mutations in braf and cdkn2a, rapidly forms subcutaneous tumors that can easily be measured by calipers to determine therapeutic efficacy. Expression of mutant amyloid precursor proteins decreases. Sk hep1 cells in culture have been shown to produce fibronectin and functionally active alpha1 protease inhibitor. In recent years many groups have established ec lines. Sk hep, skhep1, sk human liver adenocarcinoma derived from an ascites sample from a 52 year old male suffering from adenocarcinoma of the liver. Unexpected role for the human cx37 c1019t polymorphism in. We have determined that these cells are of endothelial origin. The action of transforming proteins from small dna tumor viruses seems to be remarkably similar between different viruses, as they all use prb and p53 pathways as cellular targets. The skhep1 cell line was derived from the ascetic fluid of a patient with liver adenocarcinoma.
For example, supposed thyroid lines were actually melanoma cells, supposed prostate tissue was actually bladder cancer, and supposed normal uterine cultures were actually breast cancer. These investigations of hcc cell lines provide new insights into the understanding the. These results demonstrate that, in addition to cell shape and size, adhesion expression in hepatocellular carcinoma cells is differentiation stage dependent. The skhep1 epithelial cell line was isolated from the ascitic fluid of a 52yearold caucasian male patient with adenocarcinoma of the liver in 1971 by g. Cells that are difficult to detach may be placed at 37c to facilitate dispersal. The extracellular fluid macromolecular composition. Two neuroblastoma cell lines were tested as models for neuronal cells. Many cell lines that are widely used for biomedical research have been overgrown by other, more aggressive cells. Hepatoma sk hep1 cells exhibit characteristics of oncogenic. The skhep1 cells, an immortal human cell line of endothelial origin, were obtained from dr. In addition, they constitutively produce interleukin1. Despite the location of the tumor from which sk hep1 was derived, the cell line does not have properties of hepatocytes. Surprisingly, antibody binding to the human neuroblastoma cell line sk. Name tissue cell type disease oganism age ethnicity genes expressed htb79 capan1 pancreas.